Novel UV-activated biofunctionalization of up-converting nanocrystals for detection of proteins

Abstract Lanthanide doped nanocrystals capable to emit higher energy photons under excitation with lower radiation are promising for a broad range of applications including biodetection, biosensing, and bioimaging. However, the adaptation these nanoparticles biological environment that requires good water-solubility, stability ease further functionalization still remains challenge. The application biodetection or in various assays encountered many difficulties arising mainly from strong tendency aggregation nonspecific binding. Here we present new method obtain soluble stable water-based buffers NaYF 4 :Yb 3+ Er modified surface ready conjugation biomolecules. In presented approach polyvinylpyrrolidone/vinyl alcohol copolymer (PVP/VA) photo-activatable linker (N-5-Azido-2-nitrobenzoyl group—ANB-NOS) was used initial coating due its high non-covalent affinity surface. Subsequent aminated dextran by ultraviolet light activation ANB-NOS carried out. This step has significant impact on physiological buffer as well protein conjugation. Conjugation biomolecules possible employing another photo-linker—sulfosuccinimidyl 4,4′-azipentanoate (sulfo-SDA). Bacterial Protein G been selected create universal immune-imaging agent ability bind Fc fragment most mammalian immunoglobulins. Moreover, direct attachment antibodies also examined. activity biofunctionalized tested immunoblot test, confirmed preserved attached molecules lack undesired binding precipitation assay membrane. Graphic abstract